Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Publikation bei Eurosurveillance

• Submitted: 21.1.2020
• Accepted: 22.1.2020
• Published: 23.1.2020

Bei der WHO gibt es eine früherere Version des Aufsatzes mit dem Datum 17.1.2020 und dem Hinweis, daß es sich um ein Update der Version 1 vom 13.1.2020 handelt.

Autoren

• Victor Corman
• Olfert Landt
• Marco Kaiser
• Richard Molenkamp
• Adam Meijer
• Daniel KW Chu
• Tobias Bleicker
• Sebastian Brünink
• Julia Schneider
• Marie Luisa Schmidt
• Daphne GJC Mulders
• Bart L Haagmans
• Bas van der Veer
• Sharon van den Brink
• Lisa Wijsman
• Gabriel Goderski
• Jean-Louis Romette
• Joanna Ellis
• Maria Zambon
• Malik Peiris
• Herman Goossens
• Chantal Reusken
• Marion PG Koopmans
• Christian Drosten

 

Kritik

Corman-Drosten Review Report

Virus Fragmente:

The test cannot discriminate between the whole virus and viral fragments. Therefore, the test cannot be used as a diagnostic for intact (infectious) viruses, making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus and make inferences about the presence of an infection.

Cycle Threshold:

an analytical result with a Ct value of 45 is scientifically and diagnostically absolutely meaningless (a reasonable Ct-value should not exceed 30). All this should be communicated very clearly. It is a significant mistake that the Corman-Drosten paper does not mention the maximum Ct value at which a sample can be unambiguously considered as a positive or a negative test-result. This important cycle threshold limit is also not specified in any follow-up submissions to date.

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